Biotinylated Rat CD3 epsilon&CD3 gamma Heterodimer Protein, His, Avitag™&Flag Tag (MALS verified)

T-cell surface glycoprotein CD3 delta & CD3 gamma chain, also known as CD3D & CD3G or CD3D&CD3G respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon (CD3E), CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

Product Specifications

Background

Specifications

Biotinylated Rat CD3E&CD3G Heterodimer Protein, His, Avitag&Flag Tag is produced by co-expression of CD3E and CD3G, has a calculated MW of 16.7 kDa (CD3E) and 15.7 kDa (CD3G) . Subunit CD3E carries a polyhistidine tag at the C-terminus, followed by an Avi tag and subunit CD3G is fused with a flag tag at the C-terminus. As a result of glycosylation, the protein migrates as 22-25 kDa under reducing (R) condition, and 40-45 kDa under non-reducing (NR) condition (SDS-PAGE) .

Accession Number

NP_001101610.1 (CD3E) & NP_001071114.1 (CD3G)

Expression Region

Gln 21 - Asp 103 (CD3E) & Gln 23 - Ser 116 (CD3G)

Host

HEK293

Target

CD3E & CD3G

Conjugation

Biotin-labeled

Tag

C-10xHis & C-Avi | C-Flag

Source

Rat

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.