E. coli Methionine Aminopeptidase (MAP)

<strong> E. coli Methionine Aminopeptidase (MAP)</strong>_x000D_ <strong>Catalog number:</strong> B2015148_x000D_ <strong>Lot number:</strong> Batch Dependent_x000D_ <strong>Expiration Date:</strong> Batch dependent_x000D_ <strong>Amount:</strong> 10 ug_x000D_ <strong>Molecular Weight or Concentration:</strong> 31.5 kDa (284aa)_x000D_ <strong>Supplied as:</strong> Solution_x000D_ <strong>Applications:</strong> a molecular tool for various biochemical applications_x000D_ <strong>Storage:</strong> -20 °C_x000D_ <strong>Keywords:</strong> MetAP, Peptidase M_x000D_ <strong>Grade:</strong> Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity &gt;18 MΩ-cm) and are filtered through 0.22 um._x000D_ _x000D_ <strong>References:</strong>_x000D_ 1: Wingfield PT. N-Terminal Methionine Processing Curr Protoc Protein Sci. 2017 Apr 3;88:6.14.1-6.14.3._x000D_ 2: Ben-Bassat A, Bauer K, Chang SY, Myambo K, Boosman A, Chang S. Processing of the initiation methionine from proteins: properties of the Escherichia coli methionine aminopeptidase and its gene structure J Bacteriol. 1987 Feb;169(2):751-7._x000D_ 3: Arif A, Mohammed K, Nadeem MS. Biochemical and in silico evaluation of recombinant E. coli aminopeptidase and in vitro processed human interferon α-2b Turk J Biol. 2018 Jun 13;42(3):240-249._x000D_ 4: Roderick SL, Matthews BW. Structure of the cobalt-dependent methionine aminopeptidase from Escherichia coli: a new type of proteolytic enzyme Biochemistry. 1993 Apr 20;32(15):3907-12._x000D_ 5: Chang SY, McGary EC, Chang S. Methionine aminopeptidase gene of Escherichia coli is essential for cell growth J Bacteriol. 1989 Jul;171(7):4071-2._x000D_ 6: Tsunasawa S, Izu Y, Miyagi M, Kato I. Methionine aminopeptidase from the hyperthermophilic Archaeon Pyrococcus furiosus: molecular cloning and overexpression in Escherichia coli of the gene, and characteristics of the enzyme J Biochem. 1997 Oct;122(4):843-50._x000D_ 7: You C, Lu H, Sekowska A, Fang G, Wang Y, Gilles AM, Danchin A. The two authentic methionine aminopeptidase genes are differentially expressed in Bacillus subtilis BMC Microbiol. 2005 Oct 5;5:57._x000D_ 8: Datta B. MAPs and POEP of the roads from prokaryotic to eukaryotic kingdoms Biochimie. 2000 Feb;82(2):95-107._x000D_ 9: Shapiro AB, Gao N, Thresher J, Walkup GK, Whiteaker J. A high-throughput absorbance-based assay for methionine produced by methionine aminopeptidase using S-adenosyl-L-methionine synthetase J Biomol Screen. 2011 Jun;16(5):494-505._x000D_ <a href="https://pubmed.ncbi.nlm.nih.gov/26099541">10: Rose JA, Lahiri SD, McKinney DC, Albert R, Morningstar ML, Shapiro AB, Fisher SL, Fleming PR. Novel broad-spectrum inhibitors of bacterial methionine aminopeptidase Bioorg Med Chem Lett. 2015 Aug 15;25(16):3301-6. </a>_x000D_ _x000D_ <strong>Products Related to E. coli Methionine Aminopeptidase (MAP) can be found at</strong> <a href="https://moleculardepot.com/product-category/Enzymes/"> Enzymes</a>