Recombinant Rat Endothelin-1 (Edn1), partial

Product Specifications

Product Name Alternative

Preproendothelin-1 (PPET1)

Abbreviation

Recombinant Rat Edn1 protein, partial

Gene Name

Edn1

UniProt

P22388

Expression Region

53-73aa

Organism

Rattus norvegicus (Rat)

Target Sequence

CSCSSLMDKECVYFCHLDIIW

Tag

N-terminal 6xHis-KSI-tagged

Type

In Stock Protein

Source

E.coli

Field of Research

Cancer

Relevance

Endothelins are endothelium-derived vasoconstrictor peptides.

Endotoxin

Not test

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Molecular Weight

17.8 kDa

References & Citations

"cDNA cloning, sequence analysis and tissue distribution of rat preproendothelin-1 mRNA." Sakurai T., Yanagisawa M., Inoue A., Ryan U.S., Kimura S., Mitsui Y., Goto K., Masaki T. Biochem. Biophys. Res. Commun. 175:44-47 (1991)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.