Anti-Hsp25/Hsp27 Antibody

Mouse Monoclonal Antibody specific to Hsp25/Hsp27 clone 8A7

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

HspB1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, HSP 27, Stress-responsive protein 27, SRP27

Gene Name

HSPB1

Gene ID

3315

NCBI Gene ID

UniProt

P04792

Accession Number

NP_001532.1

Cellular Locus

Cytoplasm, Nucleus, Cytoplasm, cytoskeleton, spindle

Host

Mouse

Reactivity

Human, Mouse, Rat, Bovine, Canine, Guinea Pig, Hamster

Immunogen

Hsp27 peptide

Target Antigen

Heat shock protein beta-1

Target

Hsp25/Hsp27

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, IHC, ICC/IF, IP, FCM, FACS

Field of Research

Heat Shock& Stress Proteins

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state (PubMed:10383393, PubMed:20178975). Plays a role in stress resistance and actin organization (PubMed:19166925). Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed:23728742). {PubMed:10383393, PubMed:19166925, PubMed:20178975, PubMed:23728742}.

Additionnal Information

Immunoblotting: use at 0.2ug/mL (ECL). A band of 25-27 kDa is detected. <br><br>Immunofluorescence: use at 5ug/mL. <br><br>Positive control: HeLa cell lysate

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human, mouse, rat, bovine, canine, guinea pig, and hamster Hsp 25/27. Some cross- reactivity with alpha B crystallin.

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

Hsp25 is the mouse homologue of human Hsp27, a member of the small heat shock protein family comprised of proteins of ~15->30kDa. Oligomers of Hsp27 consist of as many as 8-40 Hsp27 monomers; large oligomers have high chaperone activity whereas dimers have no chaperone activity. Hsp27 is localized to the cytoplasm of unstressed cells but redistributes to the nucleus in response to stress where it may stabilize DNA and/or the nuclear membrane. It can be rapidly phosphorylated in response to physiological stimuli, and, therefore, has been suggested as an important intermediate in second messenger- mediated signaling pathways. Hsp27 appears to be involved in cell differentiation and may play a role in termination of cell growth.