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Nile Red

Product Specifications

UNSPSC Description

Nile red (Nile blue oxazone) is a lipophilic stain. Nile red has environment-sensitive fluorescence. Nile red is intensely fluorescent in a lipid-rich environment while it has minimal fluorescence in aqueous media. Nile red is an excellent vital stain for the detection of intracellular lipid droplets by fluorescence microscopy and flow cytof uorometry. Nile red stains intracellular lipid droplets red. The fluorescence wavelength is 559/635 nm[1].

Target Antigen

Fluorescent Dye

Type

Dye Reagents

Related Pathways

Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/Nile-Red.html

Purity

98.15

Solubility

DMSO : 2 mg/mL (ultrasonic;warming;heat to 60°C)|Ethanol : 1 mg/mL (ultrasonic;warming;heat to 60°C)

Smiles

O=C1C2=CC=CC=C2C3=NC4=CC=C(N(CC)CC)C=C4OC3=C1

Molecular Weight

318.37

References & Citations

[1]Gibrán S Alemán-Nava, et al. How to use Nile Red, a selective fluorescent stain for microalgal neutral lipids. J Microbiol Methods. 2016 Sep;128:74-79.|[2]Wilber Escorcia, et al. Quantification of Lipid Abundance and Evaluation of Lipid Distribution in Caenorhabditis elegans by Nile Red and Oil Red O Staining. J Vis Exp. 2018 Mar 5;(133):57352.|[3]Elizabeth C Pino, et al. Biochemical and high throughput microscopic assessment of fat mass in Caenorhabditis elegans. J Vis Exp. 2013 Mar 30;(73):50180.|[4]Greenspan P, et al. Nile red: a selective fluorescent stain for intracellular lipid droplets. J Cell Biol. 1985 Mar;100(3):965-73.

Shipping Conditions

Room Temperature

Storage Conditions

4°C (Powder, protect from light)

Product Datasheet

http://file.medchemexpress.com/batch_PDF/HY-D0718/Nile-Red-DataSheet-MedChemExpress.pdf

Product MSDS

http://file.medchemexpress.com/batch_PDF/HY-D0718/Nile-Red-SDS-MedChemExpress.pdf

Clinical Information

No Development Reported

CAS Number

7385-67-3

Available Sizes

Curated Selection

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