Reverse Transcription Buffer (10X)

<strong>Reverse Transcription Buffer (10X)</strong>_x000D_ <table width="1028">_x000D_ <tbody>_x000D_ <tr>_x000D_ <td width="285">Catalog number:</td>_x000D_ <td width="743">B2010084</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Lot number:</td>_x000D_ <td>Batch Dependent</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Expiration date:</td>_x000D_ <td>Batch Dependent</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Volume/Weight</td>_x000D_ <td>2 mL</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>pH</td>_x000D_ <td>9</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Supplied as</td>_x000D_ <td>10 X concentrated solution. Dilute with di-water to 1X working solution.</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Appearance</td>_x000D_ <td>Clear solution</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Applications</td>_x000D_ <td>Useful for reverse transcription reactions and production of cDNA, works with AMV Reverse Transcriptase and Taq DNA polymerase. Buffer is compatible with down stream applications such as PCR.</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Shelf-Life</td>_x000D_ <td>1 year from date of manufacture</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Storage:</td>_x000D_ <td>Keep at 2-8°C</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Keywords:</td>_x000D_ <td>Reverse transcription buffer, RT Buffer, 10X RT buffer, cDNA synthesis buffer, 10X RT buffer</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Grade</td>_x000D_ <td>Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity &gt;18 MΩ-cm) and are filtered on a 0.22 um.</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>References</td>_x000D_ <td width="743">1: Tekeste SS, Wilkinson TA, Weiner EM, Xu X, Miller JT, Le Grice SF, Clubb RT,_x000D_ Chow SA. Interaction between Reverse Transcriptase and Integrase Is Required for_x000D_ Reverse Transcription during HIV-1 Replication. J Virol. 2015_x000D_ Dec;89(23):12058-69.2: Fungtammasan A, Tomaszkiewicz M, Campos-Sánchez R, Eckert KA, DeGiorgio M,_x000D_ Makova KD. Reverse Transcription Errors and RNA-DNA Differences at Short Tandem_x000D_ Repeats. Mol Biol Evol. 2016 Oct;33(10):2744-58._x000D_ _x000D_ 3: Masaki Y, Ito H, Oda Y, Yamazaki K, Tago N, Ohno K, Ishii N, Tsunoda H,_x000D_ Kanamori T, Ohkubo A, Sekine M, Seio K. Enzymatic synthesis and reverse_x000D_ transcription of RNAs incorporating 2'-O-carbamoyl uridine triphosphate. Chem_x000D_ Commun (Camb). 2016 Oct 25;52(87):12889-12892._x000D_ 4: Cosnefroy O, Murray PJ, Bishop KN. HIV-1 capsid uncoating initiates after the_x000D_ first strand transfer of reverse transcription. Retrovirology. 2016 Aug_x000D_ 22;13(1):58._x000D_ 5: Zhao LM, Li G, Gao Y, Zhu YR, Liu J, Zhu XP. Reverse transcription loop-_x000D_ mediated isothermal amplification assay for detecting tomato chlorosis virus. J_x000D_ Virol Methods. 2015 Mar;213:93-7._x000D_ 6: Chen L, Jiao Z, Liu D, Liu X, Xia Z, Deng C, Zhou T, Fan Z. One-step reverse_x000D_ transcription loop-mediated isothermal amplification for the detection of Maize_x000D_ chlorotic mottle virus in maize. J Virol Methods. 2017 Feb;240:49-53._x000D_ _x000D_ 7: Wang FX, Yuan DY, Jin YN, Hu L, Sun ZY, He Q, Zhao SH, Zhan SB, Wen YJ._x000D_ Reverse Transcription Cross-Priming Amplification-Nucleic Acid Test Strip for_x000D_ Rapid Detection of Porcine Epidemic Diarrhea Virus. <a href="https://pubmed.ncbi.nlm.nih.gov/27090105/">Sci Rep.</a> 2016 Apr_x000D_ 19;6:24702.</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Related Products</td>_x000D_ <td><a href="https://moleculardepot.com/product-category/buffers/">Biochemical Buffers and Solutions</a></td>_x000D_ </tr>_x000D_ </tbody>_x000D_ </table>