Anti-Aurora A/STK15 Antibody

EC 2.7.11.1, Aurora 2, Aurora/IPL1-related kinase 1, ARK-1, Aurora-related kinase 1, hARK1, Breast tumor-amplified kinase, Serine/threonine-protein kinase 15, Serine/threonine-protein kinase 6, Serine/threonine-protein kinase aurora-A

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, Cytoplasm, cytoskeleton, spindle pole, Cytoplasm, cytoskeleton, cilium basal body, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole, Cell projection, neuron projection

Mitotic serine/threonine kinase that contributes to the regulation of cell cycle progression (PubMed:26246606, PubMed:12390251, PubMed:18615013, PubMed:11039908, PubMed:17125279, PubMed:17360485). Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mitotic spindle, centrosome duplication, centrosome separation as well as maturation, chromosomal alignment, spindle assembly checkpoint, and cytokinesis (PubMed:26246606, PubMed:14523000). Required for normal spindle positioning during mitosis and for the localization of NUMA1 and DCTN1 to the cell cortex during metaphase (PubMed:27335426). Required for initial activation of CDK1 at centrosomes (PubMed:13678582, PubMed:15128871). Phosphorylates numerous target proteins, including ARHGEF2, BORA, BRCA1, CDC25B, DLGP5, HDAC6, KIF2A, LATS2, NDEL1, PARD3, PPP1R2, PLK1, RASSF1, TACC3, p53/TP53 and TPX2 (PubMed:18056443, PubMed:15128871, PubMed:14702041, PubMed:11551964, PubMed:15147269, PubMed:15987997, PubMed:17604723, PubMed:18615013). Regulates KIF2A tubulin depolymerase activity (PubMed:19351716). Important for microtubule formation and/or stabilization (PubMed:18056443). Required for normal axon formation (PubMed:19812038). Plays a role in microtubule remodeling during neurite extension (PubMed:19668197). Also acts as a key regulatory component of the p53/TP53 pathway, and particularly the checkpoint-response pathways critical for oncogenic transformation of cells, by phosphorylating and destabilizing p53/TP53 (PubMed:14702041). Phosphorylates its own inhibitors, the protein phosphatase type 1 (PP1) isoforms, to inhibit their activity (PubMed:11551964). Necessary for proper cilia disassembly prior to mitosis (PubMed:17604723, PubMed:20643351). Regulates protein levels of the anti-apoptosis protein BIRC5 by suppressing the expression of the SCF(FBXL7) E3 ubiquitin-protein ligase substrate adapter FBXL7 through the phosphorylation of the transcription factor FOXP1 (PubMed:28218735). {PubMed:11039908, PubMed:11551964, PubMed:12390251, PubMed:13678582, PubMed:14523000, PubMed:14702041, PubMed:15128871, PubMed:15147269, PubMed:15987997, PubMed:17125279, PubMed:17360485, PubMed:17604723, PubMed:18056443, PubMed:18615013, PubMed:19351716, PubMed:19668197, PubMed:19812038, PubMed:20643351, PubMed:26246606, PubMed:27335426, PubMed:28218735}.

Immunoblotting: 1:5,000-1:20,000 dilutionDetection of human Aurora A in HeLa and HEPG2 whole cell lysates with catalog no. 13042 at 0.2ug/mL. Detection by chemiluminescence. <br><br>Immunoprecipitation: 1-5ug/mg lysate. <br><br>These are recommended concentrations. <br><br>Endusers should determine optimal concentrations for their applications.

This antibody reacts with human Aurora kinase A, and the epitope recognized maps to a region between residues 75-125 of human Aurora kinase A. Based on 100% sequence identity this antibody is predicted to react with orangutan, gorilla, chimpanzee, and Northern white-cheeked gibbon.

Aurora kinase A, also known as serine/threonine-protein kinase 6, is an enzyme that in humans is encoded by the AURKA gene. Aurora A is a member of a family of mitotic serine/threonine kinases associated with important processes during mitosis and meiosis. Aurora A is activated by one or more phosphorylations, and its activity peaks during the G2 phase to M phase transition in the cell cycle. Aurora A dysregulation has been associated with high occurrence of cancer.