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Mouse anti Human secretory component (free and bound), conjugated with Horseradish peroxidase

Product Specifications

Background

The reactivity of the antiserum is restricted to free and bound secretory component as tested in indirect binding immunoblotting and indirect immunoperoxidase staining. To identify the presence of free or bound secretory component in Human milk, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:20 upwards; in ELISA from 1:50 upwards; in Western blotting from 1:100 upwards. These data should be interpreted as general recommendations only.

UniProt

P01833

Host

Mouse

Species Reactivity

Human

Isotype

IgG1

Clone

NI 194-4 (A89-039)

Conjugation

HRP

Type

Monoclonal Antibody

Source

Highly purified secretory component isolated from Human milk.

Applications

Dot blot, ELISA, IB, ICC, IHC

Assay Principle

ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.

Form

Purified monoclonal mouse IgG1 kappa conjugated with horseradish peroxidase, lyophilized from a solution in phosphate buffered saline (pH7,2); Nopreservative added, as it may interfere with the antibody activity; Noforeign protein added

Precautions

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.

Storage Conditions

The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C; Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product Diluted solutions should be stored at +4ºC, not refrozen, and preferably used the same day

CAS Number

9007-83-4

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