EGTA

EGTA is a specific calcium ion chelator. EGTA has an apparent calcium dissociation constant (Kd) of 60.5 nM at physiological pH (7.4) and has very high specificity for Ca2+ over Mg2+ (Mg2+ Kd 1-10 mM) . EGTA significantly inhibits the substrate adherence capacity of inflammatory macrophages[1][2].

Product Specifications

CAS Number

[67-42-5]

UNSPSC

12352200

Hazard Statement

H302, H315, H319, H335

Target

Biochemical Assay Reagents

Type

Biochemical Assay Reagents

Related Pathways

Others

Field of Research

Inflammation/Immunology

Assay Protocol

https://www.medchemexpress.com/egta.html

Purity

99.89

Solubility

1 M NaOH : ≥ 100 mg/mL|DMSO : < 1 mg/mL (ultrasonic) |H2O : 4.76 mg/mL (ultrasonic; warming; adjust pH to 9 with 1 M NaOH; heat to 60°C)

Smiles

O=C(O)CN(CC(O)=O)CCOCCOCCN(CC(O)=O)CC(O)=O

Molecular Formula

C14H24N2O10

Molecular Weight

380.35

Precautions

H302, H315, H319, H335

References & Citations

[1]Harris RA, Hanrahan JW. Effects of EGTA on calcium signaling in airway epithelial cells. Am J Physiol. 1994;267 (5 Pt 1) :C1426-C1434. doi:10.1152/ajpcell.1994.267.5.C1426|[2]Segura-Egea JJ, Jiménez-Rubio A, Rios-Santos JV, Velasco-Ortega E, Calvo-Gutierrez JR. In vitro inhibitory effect of EGTA on macrophage adhesion: endodontic implications. J Endod. 2003;29 (3) :211-213.