eIF3g Recombinant Rabbit Monoclonal Antibody [PSH09-41]
Selected Variant
HA723103
Size: 100 µL
RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
Product Specifications
CAS Number
9000-83-3
Product Name Alternative
Abbreviation
Eukaryotic translation initiation factor 3 subunit G, eIF3g, EIF3G, EIF3S4
Swiss Prot
O75821 Human
Cellular Locus
Cytoplasm, Nucleus, perinuclear region.
Host
Rabbit
Species Reactivity
Human
Immunogen
Recombinant protein within human eIF3g aa 1-320.
Isotype
IgG
Clone
PSH09-41
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, IF-Cell, FC, IP
Positive Control
K-562 cell lysate, HeLa cell lysate, SH-SY5Y cell lysate, HeLa.
Concentration
1 mg/mL
Dilution
WB: 1:25,000 ;IF-Cell: 1:100 ;FC: 1:1,000 ;IP: 1-2μg/sample
Purity
Protein A affinity purified.
Form
Liquid
Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 36 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody
Yes
Price
Frequently Asked Questions
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