Succinyl-CoA Synthetase (SCS) Activity Assay Kit
Product Specifications
Type
Colorimetric method
Field of Research
Glycolysis and Lipid Metabolism; Tricarboxylic Acid (TCA) Cycle
Assay Principle
Succinyl-CoA synthetase (SCS) is a key enzyme in the citric acid cycle and an important metabolic intermediate in the biosynthesis of porphyrins, hemes, and ketones. SCS converts succinyl-coenzyme A (CoA-SH) to succinic acid. The reaction between CoA-SH and pyruvate produces chromogenic substances with a characteristic absorption peak at 450 nm. The activity of SCS can be calculated by measuring the change of absorbance value at 450 nm.
Assay Performance Time
60 min
Sample Type
Tissue; cell
Detection Range
0.09-20.72 U/L
Sensitivity
0.09 U/L
Shipping Conditions
Ice bag
Storage Conditions
This product can be stored at -20°C for 12 months with shading light.
Inter CV
4.9-10.3%
Intra CV
2.5-8.0%
Detection Instrument
Microplate reader
Available Sizes
Frequently Asked Questions
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