Anti-Progesterone Receptor (Phospho-Ser190) Antibody

Rabbit Polyclonal Antibody specific to Progesterone Receptor (Phospho-Ser190)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-Progesterone Receptor (Phospho-Ser190) Rabbit Polyclonal Antibody

Host

Rabbit

Reactivity

Human

Immunogen

Peptide sequence that includes phosphorylation site of Serine 190 (G-L-S(p)-P-A) derived from human progesterone receptor and conjugated to KLH.

Target

Progesterone Receptor (Phospho-Ser190)

Clonality

Polyclonal

Type

Antibody

Applications

WB, IHC, IF

Field of Research

Phosphospecific Antibodies

Purification Method

Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Additionnal Information

Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~66kDa is detected. Immunofluorescence: use at dilution of 1:100-1:200. Immunohistochemistry: use at dilution of 1:50-1:100. These are recommended working dilutions. End user should determine optimal dilutions for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody detects endogenous human progesterone receptor only when phosphorylated at serine 190.Accession no.: P06401, NP_000917.3

Formulation

PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Buffer pH

pH 7.4

Target Background

Progesterone receptor (PR) is a nuclear hormone receptor of the NR3C class which includes mineralocorticoid, glucocorticoid, and androgen receptors. They exist as homodimers coupled to Hsp90 or HMGB proteins which are shed upon activation. Progesterone receptors signal directly via DNA binding and transcriptional regulation of target genes. They can also signal by binding to other proteins with transcription factors such as NF-kappa B, AP-1, or STAT. Progesterone receptor expression is induced by estrogen, and the human gene encoding the progesterone receptor has been localized to 11q22.