Anti-Bnip3L (IN) Antibody

Rabbit Polyclonal Antibody specific to Bnip3L (IN)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Adenovirus E1B19K-binding protein B5, BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A, NIP3-like protein X, NIP3L

Gene Name

BNIP3L

Gene ID

665

NCBI Gene ID

UniProt

O60238

Accession Number

NP_004322

Cellular Locus

Nucleus envelope. Endoplasmic reticulum. Mitochondrion outer membrane. Membrane

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

Peptide corresponding to aa 77- 92 of human Bnip3L (accession no. AF067396) This amino acid sequence is identical to that of mouse.

Target Antigen

BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like

Target

Bnip3L (IN)

Clonality

Polyclonal

Isotype

IgG

Type

Antibody

Applications

ELISA, WB, IHC-P, IF

Field of Research

Apoptosis

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Induces apoptosis. Interacts with viral and cellular anti-apoptosis proteins. Can overcome the suppressors BCL-2 and BCL-XL, although high levels of BCL-XL expression will inhibit apoptosis. Inhibits apoptosis induced by BNIP3. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates in mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. May function as a tumor suppressor. {PubMed:10381623, PubMed:21264228}.

Additionnal Information

Immunoblotting: use at 1ug/mL. Immunohistochemistry: use at 2ug/mL. These are recommended concentrations. Enduser should determine optimal concentrations for their applications. Positive control: Whole cell lysate of human K562 cells.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.

Specificity

This antibody recognizes monomeric human and mouse Bnip3L. A band of 40kDa is detected in immunoblots.

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

A novel BH3 domain-containing protein was recently identified and designated Bnip3L, Bnip3 , or Nix. Bnip3L is a homolog of the E1B 19K/Bcl-2 binding and pro-apoptotic protein Bnip3. Overexpression of Bnip3L induces apoptosis. Bnip3L interacts with and overcomes suppression by Bcl-2 and Bcl-xL. Bnip3L is localized in mitochondria. The messenger RNA of Bnip3L is ubiquitously expressed in human tissues. Bnip3L and Bnip3 form a new subfamily of the pro-apoptotic-mitochondrial proteins.