Anti-GABABR2 Antibody

Rabbit Polyclonal Antibody specific to GABA(B)R2

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

GABA-B receptor 2, GABA-B-R2, GABA-BR2, GABABR2, Gb2, G-protein coupled receptor 51, HG20

Gene Name

GABBR2

NCBI Gene ID

<a href="https://www.ncbi.nlm.nih.gov/gene/?term=GABBR2">GABBR2</a>

UniProt

O75899

Cellular Locus

Cell membrane, Cell junction, synapse, postsynaptic cell membrane

Host

Rabbit

Reactivity

Human

Immunogen

Synthetic peptide corresponding to aa 42-54 (TRGAPRPPPSSPP) of the rat GABABR2 subunit.

Target Antigen

γ-aminobutyric acid type B receptor subunit 2

Target

GABABR2

Clonality

Polyclonal

Type

Antibody

Applications

Field of Research

Neuroscience

Purification Method

Purified by Protein A affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Component of a heterodimeric G-protein coupled receptor for GABA, formed by GABBR1 and GABBR2 (PubMed:9872316, PubMed:9872744, PubMed:15617512, PubMed:18165688, PubMed:22660477, PubMed:24305054). Within the heterodimeric GABA receptor, only GABBR1 seems to bind agonists, while GABBR2 mediates coupling to G proteins (PubMed:18165688). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase (PubMed:10075644, PubMed:10773016, PubMed:24305054). Signaling inhibits adenylate cyclase, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipid hydrolysis (PubMed:10075644, PubMed:9872744, PubMed:10906333, PubMed:10773016). Plays a critical role in the fine-tuning of inhibitory synaptic transmission (PubMed:9872744, PubMed:22660477). Pre-synaptic GABA receptor inhibits neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA receptor decreases neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials (PubMed:9872316, PubMed:10075644, PubMed:9872744, PubMed:22660477). Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception (Probable). {PubMed:10075644, PubMed:10328880, PubMed:15617512, PubMed:18165688, PubMed:22660477, PubMed:24305054, PubMed:9872316, PubMed:9872744, ECO:0000305}.

Additionnal Information

Immunoblotting: use at 1:250 - 1:1,000 dilution. A band of ~105kDa is detected.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.

Specificity

This antibody recognizes the human and rat GABABR2 subunit.

Formulation

PBS, pH 7.4, 0.02% sodium azide.

Buffer pH

pH 7.4

Target Background

The GABAB receptor is the first discovered G-protein-coupled receptor whose function requires two subunits, R1 and R2, to form a functional receptor. The R1 subunit extracellular domain binds GABA, and the R2 subunit functions in G-protein activation.