Anti-Ceramide Kinase Antibody

Rabbit Polyclonal Antibody specific to Ceramide Kinase

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

hCERK, EC 2.7.1.138, Acylsphingosine kinase, Lipid kinase 4, LK4

Gene Name

CERK

NCBI Gene ID

UniProt

Q8TCT0

Accession Number

NP_073603.2

Cellular Locus

Cytoplasm, Cell membrane, Peripheral membrane protein

Host

Rabbit

Reactivity

Human

Immunogen

Synthetic peptide conjugated to KLH.

Target Antigen

Ceramide kinase

Target

Ceramide Kinase

Clonality

Polyclonal

Type

Antibody

Applications

IHC, IHC-P

Field of Research

Enzymes

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Catalyzes specifically the phosphorylation of ceramide to form ceramide 1-phosphate (PubMed:11956206, PubMed:16269826, PubMed:19168031). Acts efficiently on natural and analog ceramides (C6, C8, C16 ceramides, and C8-dihydroceramide), to a lesser extent on C2-ceramide and C6-dihydroceramide, but not on other lipids, such as various sphingosines (PubMed:11956206, PubMed:16269826, PubMed:19168031). Shows a greater preference for D-erythro isomer of ceramides (PubMed:16269826). Binds phosphoinositides (PubMed:19168031). {PubMed:11956206, PubMed:16269826, PubMed:19168031}.

Additionnal Information

<br><br>Immunohistochemistry: Use at 20ug/ml.

Storage Conditions

This antibody is stable at 4°C (short- term) and -70°C (long-term).

Specificity

This antibody recognizes the N-terminal extracellular domain of human CERK.

Formulation

PBS containing 0.1% sodium azide

Target Background

Ceramide kinase (CERK), which phosphorylates ceramide to produce ceramide 1-phosphate (C1P), was first described as a Ca+2-stimulated lipid kinase that copurified with brain synaptic vesicles. CERK activity has been reported in HL60 cells, mast cells, and neutrophils. CERK is involved in phagolysosome formation in plymorphonuclear leukocytes and also in liposome fusion. C1P has been reported to have mitogenic effects. More recently, C1P was found to induce arachidonic acid release and prostanoid synthesis.