SingleFlowEx CD15 APC
SingleFlowEx is fluorochrome-labeled monoclonal antibody single reagent for use in laboratory-developed multicolor flow cytometry tests in order to examine human clinical samples. SingleFlowEx can be used in various multicolor combinations. Laboratory shall establish the SingleFlowEx performance characteristics in conjunction with other reagents in normal and pathological samples.
Product Specifications
Certification
RUO (GMP)
Clone
MEM-158
Applications
FC (QC tested)
Concentration
0.050 mg/mL
Format
APC
Buffer
Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Components
1 vial of monoclonal antibody reagent.The content (1 mL) is sufficient for min. 100 Tests.
References & Citations
*Sutherland DR, Richards SJ, Ortiz F, Nayyar R, Benko M, Marinov I, Illingworth A: CD71 improves delineation of PNH type III, PNH type II, and normal immature RBCS in patients with paroxysmal nocturnal hemoglobinuria. Cytometry B Clin Cytom. 2019 Nov 8. doi: 10.1002/cyto.b.21853. , URL: https://www.ncbi.nlm.nih.gov/pubmed/31705743, *Sutherland DR, Illingworth A, Marinov I, Ortiz F, Andreasen J, Payne D, Wallace PK, Keeney M: ICCS/ESCCA consensus guidelines to detect GPI-deficient cells in paroxysmal nocturnal hemoglobinuria (PNH) and related disorders part 2 - reagent selection and assay optimization for high-sensitivity testing. Cytometry B Clin Cytom. 2018 Jan;94 (1) :23-48., URL: https://www.ncbi.nlm.nih.gov/pubmed/29236353, *Sutherland DR, Ortiz F, Quest G, Illingworth A, Benko M, Nayyar R, Marinov I: High-sensitivity 5-, 6-, and 7-color PNH WBC assays for both Canto II and Navios platforms. Cytometry B Clin Cytom. 2018 Jul;94 (4) :637-651., URL: https://www.ncbi.nlm.nih.gov/pubmed/29381839 , *Use of this product has not been published., URL:
Other References
*Benharroch D, Dima E, Levy A, Ohana-Malka O, Ariad S, Prinsloo I, Mejirovsky E, Sacks M, Gopas J: Differential expression of sialyl and non-sialyl-CD15 antigens on Hodgkin-Reed-Sternberg cells: significance in Hodgkin's disease. Leuk Lymphoma. 2000 Sep;39 (1-2) :185-94., URL: https://pubmed.ncbi.nlm.nih.gov/10975398/, *Hakomori S: Le (X) and related structures as adhesion molecules. Histochem J. 1992 Nov;24 (11) :771-6., URL: https://pubmed.ncbi.nlm.nih.gov/1362193/, *Li C, Wong P, Pan T, Xiao F, Yin S, Chang B, Kang SC, Ironside J, Sy MS: Normal cellular prion protein is a ligand of selectins: binding requires Le (X) but is inhibited by sLe (X) . Biochem J. 2007 Sep 1;406 (2) :333-41., URL: https://pubmed.ncbi.nlm.nih.gov/17497959/
Storage Conditions
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
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