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Human Lipoprotein Lipase Protein, His Tag (active enzyme)

Key enzyme in triglyceride metabolism. Catalyzes the hydrolysis of triglycerides from circulating chylomicrons and very low density lipoproteins (VLDL), and thereby plays an important role in lipid clearance from the blood stream, lipid utilization and storage. Although it has both phospholipase and triglyceride lipase activities it is primarily a triglyceride lipase with low but detectable phospholipase activity. Mediates margination of triglyceride-rich lipoprotein particles in capillaries. Recruited to its site of action on the luminal surface of vascular endothelium by binding to GPIHBP1 and cell surface heparan sulfate proteoglycans.

Product Specifications

Background

Key enzyme in triglyceride metabolism. Catalyzes the hydrolysis of triglycerides from circulating chylomicrons and very low density lipoproteins (VLDL), and thereby plays an important role in lipid clearance from the blood stream, lipid utilization and storage. Although it has both phospholipase and triglyceride lipase activities it is primarily a triglyceride lipase with low but detectable phospholipase activity. Mediates margination of triglyceride-rich lipoprotein particles in capillaries. Recruited to its site of action on the luminal surface of vascular endothelium by binding to GPIHBP1 and cell surface heparan sulfate proteoglycans.

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 52.3 kDa. The protein migrates as 55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

Accession Number

P06858-1

Expression Region

Ala 28 - Gly 475

Host

CHO

Target

Lipoprotein Lipase

Conjugation

Unconjugated

Tag

C-10xHis

Source

Human

Stability

The product MUST be stored at -70°C or lower upon receipt; -70°C for 3 months under sterile conditions.

Endotoxin

1.0 EU per μg

Purity

85%

Format

Liquid

Buffer

50 mM Tris, 500 mM NaCl, 5 mM CHAPS, pH7.5

Additives

Glycerol

Molecular Weight

52.3 kDa

Additionnal Information

Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments

Shipping Conditions

Dry ice

Storage Conditions

-70°C

Package Size

1mg*1

Host or Source

CHO

Species

Human

Protein ID

P06858-1

Preservative

Glycerol

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