SARS-CoV-2 (COVID-19) Nucleocapsid protein, His Tag
SARS-CoV-2 Nucleocapsid protein, His Tag (NUN-C51H2) is expressed from E. coli cells.
Product Specifications
Background
Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology; 517: 62-68) . The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.
Specifications
This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 51.0 kDa. The protein migrates as 53-55 kDa under reducing (R) condition (SDS-PAGE) .
Accession Number
QHO62115.1
Expression Region
Met 1 - Ala 419
Host
E. coli
Target
Nucleocapsid protein
Conjugation
Unconjugated
Tag
N-6xHis
Source
SARS-CoV-2
Stability
● The product MUST be stored at -70°C or lower upon receipt; ● -70°C for 3 months under sterile conditions.
Endotoxin
1.0 EU per μg
Purity
90%
Bioactivity
Immobilized SARS-CoV-2 Nucleocapsid protein, His Tag (Cat. No. NUN-C51H2) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Nucleocapsid Antibody, Chimeric mAb, Human IgG1 (Cat. No. NUN-CH15) with a linear range of 0.1-3 ng/mL (QC tested).
Format
Liquid
Buffer
10 mM PB, Arginine, pH7.4
Molecular Weight
51.0 kDa
Shipping Conditions
Dry ice
Storage Conditions
-70°C
Package Size
5mg*2
Product Datasheet
Https://www.acrobiosystems.com/static/main/products/pdf/ds/NUN-C51H2.pdf
Species
SARS-CoV-2
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