2',7'-Dichlorofluorescein

2',7'-Dichlorofluorescein is an important fluorescent product in the detection of oxidative stress and can be used to measure reactive oxygen species (ROS) (Ex=503 nm and Em=523 nm) [1][2].

Product Specifications

CAS Number

[76-54-0]

UNSPSC

12171500

Hazard Statement

H315, H319

Target

Fluorescent Dye; Reactive Oxygen Species (ROS)

Type

Dye Reagents

Related Pathways

Immunology/Inflammation; Metabolic Enzyme/Protease; NF-κB; Others

Applications

Cancer-programmed cell death

Field of Research

Inflammation/Immunology

Assay Protocol

https://www.medchemexpress.com/2-7-dichlorofluorescein.html

Purity

99.77

Solubility

DMSO : 125 mg/mL (ultrasonic)

Smiles

O=C1OC2(C3=C(OC4=C2C=C(Cl)C(O)=C4)C=C(O)C(Cl)=C3)C5=C1C=CC=C5

Molecular Formula

C20H10Cl2O5

Molecular Weight

401.20

Precautions

H315, H319

References & Citations

[1]Reiniers MJ, et al. Preparation and Practical Applications of 2',7'-Dichlorodihydrofluorescein in Redox Assays. Anal Chem. 2017 Apr 4;89 (7) :3853-3857. |[2]Xiuping Chen, et al. 2',7'-Dichlorodihydrofluorescein as a fluorescent probe for reactive oxygen species measurement: Forty years of application and controversy. Free Radic Res. 2010 Jun;44 (6) :587-604.