Human SMAC / Diablo Protein, His Tag
Human SMAC, His Tag (DIO-H5129) is expressed from E. coli cells. It contains AA Ala 56 - Asp 239 (Accession # NP_063940) .
Product Specifications
Background
Diablo homolog, mitochondrial (DIABLO) is also known as direct IAP-binding protein with low pI and second mitochondria-derived activator of caspase (SMAC), which can interact with BIRC2/c-IAP1, BIRC3/c-IAP2, XIAP/BIRC4, BIRC6/bruce and BIRC7/livin. Acting by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP), DIABLO can also promote apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Also, DIABLO inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Furthermore, DIABLO is defective in the capacity to down-regulate the XIAP/BIRC4 abundance.
Specifications
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 21.7 kDa. The protein migrates as 22 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) .
Accession Number
NP_063940
Expression Region
Ala 56 - Asp 239
Host
E. coli
Target
SMAC
Conjugation
Unconjugated
Tag
C-6xHis
Source
Human
Stability
● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin
1.0 EU per μg
Purity
95%
Format
Powder
Buffer
20 mM HEPES, 150 mM NaCl, pH7.5
Additives
Trehalose
Molecular Weight
21.7 kDa
Shipping Conditions
RT
Storage Conditions
-20°C
Package Size
200ug*1
Product Datasheet
Https://www.acrobiosystems.com/static/main/products/pdf/ds/DIO-H5129.pdf
Species
Human
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