Recombinant Human TNFA

Product Specifications

Product Name Alternative

APC1, APC1 protein, Cachectin, DIF, Differentiation inducing factor, Macrophage cytotoxic factor, Tnf, TNF superfamily member 2, TNF superfamily, member 2, TNF

UniProt

P01375

Expression Region

77-233

Host

E.Coli

Tag

N-terminal His Tag

Applications

Western Blot, ELISA

Field of Research

Immunology, Cardiovascular, Developmental biology, Signal Transduction

Endotoxin

< 1 EU/µg as determined by LAL test.

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin. The ED50 for this effect is 0.1-0.3 ng/mL.

Form

Lyophilized powder

Reconstitution

Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/ml by gently pipetting 2-3 times, don't vortex.

Molecular Weight

17.2 kDa

Shipping Conditions

4°C with ice bag

Storage Conditions

The lyophilized protein is stable at -20 °C for up to 1 year. For extended storage, it is recommended to further dilute in working aliquots after reconstitution. The protein solution is stable at ≤ -20 °C for 3 months, or 2-7 days at 2-8 °C under sterile conditions.Avoid repeated freeze/thaw cycle.