Recombinant Human TNFA
Product Specifications
Product Name Alternative
APC1, APC1 protein, Cachectin, DIF, Differentiation inducing factor, Macrophage cytotoxic factor, Tnf, TNF superfamily member 2, TNF superfamily, member 2, TNF
UniProt
P01375
Expression Region
77-233
Host
E.Coli
Tag
N-terminal His Tag
Applications
Western Blot, ELISA
Field of Research
Immunology, Cardiovascular, Developmental biology, Signal Transduction
Endotoxin
< 1 EU/µg as determined by LAL test.
Purity
Greater than 90% as determined by SDS-PAGE.
Activity
Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin. The ED50 for this effect is 0.1-0.3 ng/mL.
Form
Lyophilized powder
Reconstitution
Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/ml by gently pipetting 2-3 times, don't vortex.
Molecular Weight
17.2 kDa
Shipping Conditions
4°C with ice bag
Storage Conditions
The lyophilized protein is stable at -20 °C for up to 1 year. For extended storage, it is recommended to further dilute in working aliquots after reconstitution. The protein solution is stable at ≤ -20 °C for 3 months, or 2-7 days at 2-8 °C under sterile conditions.Avoid repeated freeze/thaw cycle.
Formulation
Lyophilized from a 0.2 μm filtered solution of 10 mM Hepes, 150 mM NaCl with 5% trehalose, pH 7.4.
Organism Species
Human
Available Sizes
Frequently Asked Questions
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