Recombinant Mouse Transmembrane protease serine 11A (Tmprss11a), partial

Product Specifications

Product Name Alternative

(Airway trypsin-like protease 1) (Serine protease DESC3) (DESC-3)

Abbreviation

Recombinant Mouse Tmprss11a protein, partial

Gene Name

Tmprss11a

UniProt

Q3UQ41

Expression Region

45-389aa

Organism

Mus musculus (Mouse)

Target Sequence

QVNSNSGQKSSNQLKDLQETNENLVDEIFIDSALNNRYIKNHVVGLTPEEDDTKADIVMVFQPPATGRRTVGKKTHHSILDQKTRNARALPADVSLVQVKDCGKRAIPLIANRIVSGNPAAKGAWPWQVSLQRSNIHQCGGTLIGNMWVVTAAHCFRTNSNPRQWTLSFGTTINPPLMKRDVRRIIMHERYRPPARDHDIALVQFSPRVTFSDEVRRICLPEPSASFPPNSTVYITGFGALYYGGESQNELREARVQIISNDICKKRHVYGNEIKRGMFCAGFLEGNYDACRGDSGGPLVIRDNKDTWYLIGIVSWGDNCGQKNKPGVYTQVTYYRHWIASKTGL

Tag

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Type

Developed Protein

Source

E.coli

Field of Research

Cell Biology

Relevance

Probable serine protease which may play a role in cellular senescence. Overexpression inhibits cell growth and induce G1 cell cycle arrest.

Endotoxin

Not test

Purity

Greater than 85% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Molecular Weight

46.2 kDa

References & Citations

"Mouse DESC1 is located within a cluster of seven DESC1-like genes and encodes a type II transmembrane serine protease that forms serpin inhibitory complexes." Hobson J.P., Netzel-Arnett S., Szabo R., Rehault S.M., Church F.C., Strickland D.K., Lawrence D.A., Antalis T.M., Bugge T.H. J. Biol. Chem. 279:46981-46994 (2004)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.