MLKL (phospho Ser358) rabbit pAb

This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2015],

Product Specifications

Background

This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF) -induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2015]

Product Name Alternative

Mixed lineage kinase domain-like protein

UniProt

Q8NB16

Swiss Prot

Q8NB16

Reactivity

Human

Immunogen

Synthesized peptide derived from human MLKL (phospho S358)

Target

MLKL

Clonality

Polyclonal

Source

Rabbit

Applications

IHC; IF; WB

Concentration

1 mg/ml

Dilution

IHC-p 1:100-500; IF/ICC 1:100-500; WB 1:500-2000

Buffer

-20°C/1 year

Molecular Weight

55kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

55kD

Fragment

IgG

Subcellular Location

Cytoplasm . Cell membrane . Nucleus . Localizes to the cytoplasm and translocates to the plasma membrane on necroptosis induction (PubMed:24316671) . Localizes to the nucleus in response to orthomyxoviruses infection (By similarity) . .