Bat coronavirus BM48-31/BGR/2008 Spike Trimer Protein (R672A, KV972-973PP), His Tag

Bat coronavirus BM48-31/BGR/2008 Spike Trimer, His Tag (SPN-B52H9) is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. It contains AA Asn 14 - Pro 1199 (Accession # E0XIZ3 (R672A, KV972-973PP) ) .

Product Specifications

Background

The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 136.5 kDa. The protein migrates as 160-190 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

E0XIZ3 (R672A, KV972-973PP)

Expression Region

Asn 14 - Pro 1199

Host

HEK293

Target

Spike protein

Conjugation

Unconjugated

Tag

C-10xHis

Source

Bat coronavirus

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.