Rat Integrin alpha 1 beta 1 (ITGA1&ITGB1) Heterodimer Protein, His Tag&Tag Free
Rat ITGA1&ITGB1 Heterodimer Protein, His Tag&Tag Free (IT1-R52W7) is expressed from human 293 cells (HEK293) . It contains AA Phe 29 - Pro 1142 (ITGA1) & Gln 21 - Asp 729 (ITGB1) (Accession # P18614-1 (ITGA1) & P49134-1 (ITGB1) ) .
Product Specifications
Background
Integrin alpha-1/beta-1 is a receptor for laminin and collagen. The Integrin alpha-1/beta-1 and Integrin alpha-2/beta-1 integrins provide critical support for vascular endothelial growth factor signaling, endothelial cell migration, and tumor angiogenesis. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Involved in anchorage-dependent, negative regulation of EGF-stimulated cell growth.
Specifications
Rat ITGA1&ITGB1 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGA1 and ITGB1, has a calculated MW of 130.0 kDa (ITGA1) and 83.7 kDa (ITGB1) . Subunit ITGA1 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus.
Accession Number
P18614-1 (ITGA1) & P49134-1 (ITGB1)
Expression Region
Phe 29 - Pro 1142 (ITGA1) & Gln 21 - Asp 729 (ITGB1)
Host
HEK293
Target
Integrin alpha 1 beta 1
Conjugation
Unconjugated
Tag
C-10xHis | Native
Source
Rat
Stability
● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin
1.0 EU per μg
Purity
95%
Bioactivity
Immobilized Human Collagen I protein at 2 μg/mL (100 μL/well) can bind Rat ITGA1&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-R52W7) with a linear range of 0.02-0.313 μg/mL (QC tested).
Format
Powder
Buffer
50 mM Tris, 150 mM NaCl, pH7.5
Additives
Trehalose
Molecular Weight
130 kDa (ITGA1) & 83.7 kDa (ITGB1)
Shipping Conditions
RT
Storage Conditions
-20°C
Package Size
200ug*5
Product Datasheet
Https://www.acrobiosystems.com/static/main/products/pdf/ds/IT1-R52W7.pdf
Species
Rat
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