Sheep anti Human Guanalyate Kinase GKWW domain (MAGI)

Membrane-associated guanylate kinase with inverted orientation (MAGI3) is a novel, 130 kDa guanylate kinase which is part of a family of multi-PDZ domain containing guanylate kinases. These guanylate kinases are localized to epithelial cell tight junctions. MAGI3 and PTEN/MMAC cooperate to modulate the kinase activity of AKT/protein kinase B (PKB). MAGI3 positions the PTEN/MMAC phosphatase to specific subcellular locations that are involved with the regulation of cell proliferation and survival. MAGI3, as well as other proteins in this family, are made up of an N-terminal guanylate kinase domain, followed by a WW domain, named for two conserved tryptophan residues and first identified as two repeats in mouse YAP65, which is then followed by 6 PDZ domains.

Antibody detects MAGI-3 by Western blot at 1-10 µg/mL. Detects a 130 kDa band in cells transfected with full-length MAGI-3 protein. Optimal concentration should be evaluated by serial dilutions.

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.

1. Wu, Y., et al. Interaction of the tumor suppressor PTEN/MMAC with a PDZ domain of MAGI3, a novel membrane-associated guanylate kinase. J. Biol. Chem. 2000, 275, 21477-21485