Anti-Hsp70 Antibody

Mouse Monoclonal Antibody specific to Hsp70 clone 3A3

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-Hsp70 clone 3A3 Mouse Monoclonal Antibody

Gene ID

3303

UniProt

P08107

Accession Number

NP_005336.3

Host

Mouse

Reactivity

Human, Mouse, Rat, Fish, Avian, Amphibian, Drosophila, Yeast

Immunogen

Recombinant human Hsp70 expressed in E. coli.

Target

Hsp70

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, IHC, ICC/IF, IP, AM

Field of Research

Heat Shock& Stress Proteins

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Additionnal Information

Immunoblotting: use at 0.2ug/mL (ECL). A band of ~70 kDa is detected. <br><br>Immunoprecipitation: use at 1-2ug/mLImmunohistochemistry/Immunocyto- chemistry: use at 2 ug/mLUser should determine optimal concentrations for their applications. <br><br>Positive control: Heat-shocked HeLa cell lysate.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human, mouse, rat, fish, avian, amphibian, Drosophila, and yeast Hsp70, Hsc70, p75, and, following heat shock, Hsp72.

Formulation

PBS, pH 7.4 and 50% glycerol.

Buffer pH

pH 7.4

Target Background

Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides. Hsp70 recognizes and binds to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound protein. The ability of Hsp70 to undergo cycles of binding and release from hydrophobic stretches of partially unfolded proteins is the basis for their role in a variety of intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport.