Recombinant Hordeum vulgare B3-hordein

Product Specifications

Abbreviation

Recombinant Hordeum vulgare B3-hordein protein

UniProt

P06471

Expression Region

1-264aa

Organism

Hordeum vulgare (Barley)

Target Sequence

QQPVSRQPQQIIPQQPQQPFPLQPQQPQPFPQQPIPQQPQPYPQQPQSFPQQPFPSQQPFPQQPPFWQQQPVLSQQQPCTQDQTPLLQEQQDQMLVQVQIPFVHPSILQQLNPCKVFLQQQCSPLAMSQRIARSQMLQQSSCHVLQQQCCQQLPQIPEQLRHEAVRAIVYSIVLQEQSLQLVQGVSQPQQQSQQQQVGQCSFQQPQPQQGQQQQVPQSVFLQPHQIAQLEATTSIALRTLPTMCSVNVPLYRIVPLAIDTRVGV

Tag

N-terminal 6xHis-tagged

Type

Developed Protein

Source

E.coli

Field of Research

Signal Transduction

Relevance

Sulfur-rich seed storage protein.

Endotoxin

Not test

Purity

Greater than 85% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Molecular Weight

36.2 kDa

References & Citations

"Separation and characterization of basic barley seed proteins." Kristoffersen H.E., Flengsrud R. Electrophoresis 21:3693-3700 (2000)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.