Anti-α 1 GABA-A Receptor Antibody

Ligand-gated chloride channel which is a component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the brain (PubMed:23909897, PubMed:25489750, PubMed:29950725). Plays an important role in the formation of functional inhibitory GABAergic synapses in addition to mediating synaptic inhibition as a GABA-gated ion channel (PubMed:23909897, PubMed:25489750). The gamma2 subunit is necessary but not sufficient for a rapid formation of active synaptic contacts and the synaptogenic effect of this subunit is influenced by the type of alpha and beta subunits present in the receptor pentamer (By similarity). The alpha1/beta2/gamma2 receptor and the alpha1/beta3/gamma2 receptor exhibit synaptogenic activity (PubMed:23909897, PubMed:25489750). GABRA1-mediated plasticity in the orbitofrontal cortex regulates context-dependent action selection (By similarity). Functions also as histamine receptor and mediates cellular responses to histamine (By similarity). {UniProtKB:P62812, UniProtKB:P62813, PubMed:23909897, PubMed:25489750, PubMed:29950725}.

Immunoblotting: use at 1-10ug/mL. A band of ~55kDa is detected.Immunohistochemistry and <br><br>Immunocytochemistry: use at 0.1-1ug/mL <br><br>Immunofluorescence: use at 1-10ug/mL <br><br>These are recommended concentrations. User should determine optimal concentrations for their application. <br><br>Positive control: Rat brain lysate.

GABA is the major inhibitory neuro- transmitter in the vertebrate brain. GABA receptors consist of five subunits that form a chloride ion channel. Subunit families alpha, beta, g, and delta have been studied extensively; π and epsilon have been identified recently. Studies of recombinant GABA receptors have shown that individual subunits and their subtypes confer different sensitivities to GABA receptor modulators. Subunit subtypes are differentially expressed throughout development and in different CNS regions, reducing the total number of possible isoforms that can be formed in different brain regions and in individual cells.