Anti-α 1 GABA-A Receptor Antibody

Mouse Monoclonal Antibody specific to α 1 GABA-A Receptor

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

GABA(A receptor subunit α-1

Gene Name

GABRA1

NCBI Gene ID

<a href="https://www.ncbi.nlm.nih.gov/gene/?term=GABRA1">GABRA1</a>

UniProt

P14867

Cellular Locus

Cell junction, synapse, postsynaptic cell membrane, Cell membrane, Cytoplasmic vesicle membrane

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Fusion protein corresponding to aa 355-394 of mouse Alpha 1 GABA-A receptor (accession no. P62812).

Target Antigen

γ-aminobutyric acid receptor subunit α-1

Target

α 1 GABA-A Receptor

Clonality

Monoclonal

Isotype

IgG2a

Type

Antibody

Applications

WB, IHC, ICC/IF, AM

Field of Research

Neuroscience

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Ligand-gated chloride channel which is a component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the brain (PubMed:23909897, PubMed:25489750, PubMed:29950725). Plays an important role in the formation of functional inhibitory GABAergic synapses in addition to mediating synaptic inhibition as a GABA-gated ion channel (PubMed:23909897, PubMed:25489750). The gamma2 subunit is necessary but not sufficient for a rapid formation of active synaptic contacts and the synaptogenic effect of this subunit is influenced by the type of alpha and beta subunits present in the receptor pentamer (By similarity). The alpha1/beta2/gamma2 receptor and the alpha1/beta3/gamma2 receptor exhibit synaptogenic activity (PubMed:23909897, PubMed:25489750). GABRA1-mediated plasticity in the orbitofrontal cortex regulates context-dependent action selection (By similarity). Functions also as histamine receptor and mediates cellular responses to histamine (By similarity). {UniProtKB:P62812, UniProtKB:P62813, PubMed:23909897, PubMed:25489750, PubMed:29950725}.

Additionnal Information

Immunoblotting: use at 1-10ug/mL. A band of ~55kDa is detected.Immunohistochemistry and Immunocytochemistry: use at 0.1-1ug/mL Immunofluorescence: use at 1-10ug/mL These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Rat brain lysate.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human, mouse, and rat Alpha 1 GABA-A receptor. It does not cross-react with Alpha 2 or Alpha 3 GABA-A receptors.

Formulation

PBS, pH 7.4; 50% glycerol, 0.09% sodium azide. Purified by Protein G affinity chromatography.

Buffer pH

pH 7.4

Target Background

GABA is the major inhibitory neuro- transmitter in the vertebrate brain. GABA receptors consist of five subunits that form a chloride ion channel. Subunit families alpha, beta, g, and delta have been studied extensively; Ï€ and epsilon have been identified recently. Studies of recombinant GABA receptors have shown that individual subunits and their subtypes confer different sensitivities to GABA receptor modulators. Subunit subtypes are differentially expressed throughout development and in different CNS regions, reducing the total number of possible isoforms that can be formed in different brain regions and in individual cells.