Anti-BK Beta3a K+ Channel Antibody

Mouse Monoclonal Antibody specific to BK Beta3a K+ Channel

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

BK channel subunit beta-3, BKbeta3, Hbeta3, Calcium-activated potassium channel, subfamily M subunit beta-3, Charybdotoxin receptor subunit beta-3, K(VCAbeta-3, Maxi K channel subunit beta-3, Slo-beta-3

Gene Name

KCNMB3

NCBI Gene ID

<a href="https://www.ncbi.nlm.nih.gov/gene/?term=KCNMB3">KCNMB3</a>

UniProt

Q9NPA1

Accession Number

NP_055222.3

Cellular Locus

Membrane; Multi-pass membrane protein.

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Fusion protein corresponding to aa 1-49 (unique N-terminus) of mouse BK Beta3a (accession no. XP_989315). This sequence is 86

Target Antigen

Calcium-activated potassium channel subunit beta-3

Target

BK Beta3a K+ Channel

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Field of Research

Ion Channels

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Regulatory subunit of the calcium activated potassium KCNMA1 (maxiK) channel. Modulates the calcium sensitivity and gating kinetics of KCNMA1, thereby contributing to KCNMA1 channel diversity. Alters the functional properties of the current expressed by the KCNMA1 channel. Isoform 2, isoform 3 and isoform 4 partially inactivate the current of KCNBMA. Isoform 4 induces a fast and incomplete inactivation of KCNMA1 channel that is detectable only at large depolarizations. In contrast, isoform 1 does not induce detectable inactivation of KCNMA1. Two or more subunits of KCNMB3 are required to block the KCNMA1 tetramer. {PubMed:10766764, PubMed:10864947}.

Additionnal Information

Immunoblotting: use at 1ug/mL. A band of ~32kDa is detected.Immunohistochemistry and Immunocytochemistry: use at 0.1-1ug/mL Immunofluorescence: use at 1-10ug/mL These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Adult rat brain lysate

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human, mouse, and rat BK Beta3a. It does not react with BK Beta1, Beta2, Beta3b or Beta4.

Formulation

PBS, pH 7.4; 50% glycerol, 0.09% sodium azide.

Buffer pH

pH 7.4

Target Background

Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient. BK channels contribute to electrical impulses, proper signal transmission, and regulation of neurotransmitter release. Research suggests that distribution of the beta subunits in the brain can modulate the BK channels to contribute to the patho- physiology of epilepsy and dyskinesia.