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Mouse IL-25 Protein, His tag (Animal-Free)

Interleukin 25 (IL-25) is a cytokine with proinflammatory and anti-inflammatory effects, it predicts a molecular mass of 16.7 kDa. It can induce NF-κB activation, and stimulate the production of IL-8, which is the major chemotactic substance of neutrophils.

Product Specifications

Background

Interleukin 25 (IL-25) is a cytokine with proinflammatory and anti-inflammatory effects, it predicts a molecular mass of 16.7 kDa. It can induce NF-κB activation, and stimulate the production of IL-8, which is the major chemotactic substance of neutrophils.

Product Name Alternative

IL-17E, Il17e

Accession Number

NP_542767 1

Tag

IL-25

Type

Cytokines and proteins

Applications

His tag

Purity

>98% as determined by SDS-PAGE.

Activity

Measured by its ability to induce CXCL1 secretion in HT‑ 29 cells.The ED50 for this effect is <1 ng/mL

Molecular Weight

18.41 kDa

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Features & Benefits

Endotoxin: <0.1 EU per 1 μg of the protein by the LAL method.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA) . Please prevent freeze-thaw cycles

Protein Length

The recombinant Mouse IL-25 consists of 152 amino acids and predicts a molecular mass of 18.41 kDa

Formulation

Lyophilized from 20 mM sodium citrate, 0.2 M NaCl, pH 4.5

Applications Notes

Mouse

Other Product Names

IL-17E, Il17e

Recommended Usage

Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously

Preparation

E. coli

AA Sequence

Amino acid sequence derived from Mouse IL-25 (Val 17-Ala 169) (NP_542767.1) was expressed with 6xHis tag at the C-terminus

Available Sizes

Curated Selection

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