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Monkeypox virus (Democratic Republic of the Congo) B6R Protein, His Tag (MALS verified)

Monkeypox virus (Democratic Republic of the Congo) B6R Protein, His Tag (B6R-M52H3) is expressed from human 293 cells (HEK293) . It contains AA Thr 20 - His 279 (Accession # EPI_ISL_19093812, GISAID) .

Product Specifications

Background

Monkeypox is a rare zoonosis caused by monkeypox virus, which has become the most serious orthpoxvirus and consists of complex double stranded DNA. The pathogenesis of monkeypox is that the virus invades the body from respiratory mucosa, multiplies in lymphocytes, and incurs into blood producing transient venereal toxemia. after the virus multiplies in cells, the cells can invade the blood and propagate to the skin of the whole body, causing lesions. B6R, located both on the membranes of infected cells and on the enveloped virion (EV) envelope, is similar to the complement control protein-like B5R of the vaccinia virus.

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 30.7 kDa. The protein migrates as 40-43 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

EPI_ISL_19093812

Expression Region

Thr 20 - His 279

Host

HEK293

Target

B6R (MPXV)

Conjugation

Unconjugated

Tag

C-10xHis

Source

Monkeypox virus (Democratic Republic of the Congo)

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Monkeypox virus (Democratic Republic of the Congo) B6R Protein, His Tag (Cat. No. B6R-M52H3) at 1 μg/mL (100 μL/well) can bind Anti-Monkeypox virus/MPXV B6R/SL-159 Polyclonal Antibody with a linear range of 0.2-8 ng/mL (QC tested).

Format

Powder

Buffer

PBS, pH7.4

Additives

Trehalose

Molecular Weight

30.7 kDa

Additionnal Information

Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments

Shipping Conditions

RT

Storage Conditions

-20°C

Package Size

50ug*1

Host or Source

HEK293

Species

Monkeypox virus (Democratic Republic of the Congo)

Protein ID

EPI_ISL_19093812

Preservative

Trehalose

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