Anti-Methylated Lysine Antibody
Rabbit Polyclonal Antibody specific to Methylated Lysine
Product Specifications
CAS Number
9007-83-4
Product Name Alternative
Anti-Methylated Lysine Rabbit Polyclonal Antibody
Host
Rabbit
Reactivity
All
Immunogen
Methylated lysine-KLH conjugate.
Target
Methylated Lysine
Clonality
Polyclonal
Type
Antibody
Applications
WB, ICC/IF, IP, ELISA
Field of Research
Neuroscience
Purification Method
Purified by Protein A affinity chromatography
Concentration
1.0 mg/mL
Dilution
Dilute in PBS or medium that is identical to that used in the assay system.
Format
Purified
Form
Liquid
Buffer
Phosphate Buffered Saline
Additionnal Information
Immunoblotting: use at 0.5-1.0ug/
<br><br>mLELISA: use at 1-10ug/mL with methylated lysine-containing proteins on the solid phase.
<br><br>These are recommended concentrations.
<br><br>Enduser should determine optimal concentrations for their applications.
Storage Conditions
This product is stable for at least 1 year at -20°C. Freeze in multiple aliquots to avoid repeated freeze-thaw cycles.
Specificity
This antibody recognizes proteins containing methylated lysine residues. It does not recognize non-methylated proteins.
Formulation
PBS, pH 7.0, and 50% glycerol.
Buffer pH
pH 7.0
Target Background
Post-translational modifications of proteins play critical roles in the regulation and function of many biological processes. A common post-transcriptional modification of lysine is methylation. Lysine can be methylated once, twice, or three times by lysine methyltransferases. The transfer of methyl groups from S-adenosyl methionine to histones is catalyzed by enzymes known as histone methyltransferases. Histones that are methylated on certain residues can repress or activate gene expression.
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