Chicken anti FLAG Epitope (DYKDDDDK)

Chickens were immunized with FLAG Epitope (DYKDDDDK) conjugated to KLH. After multiple immunizations, eggs were collected and the immunoglobulin fraction prepared. Antibodies were immunoaffinity purified using the peptide immobilized on a solid support.

Antibody was analyzed using amino-terminal Met-Flag-BAP, amino- terminal FLAG-BAP and carboxy-terminal FLAG-BAP fusion proteins and Invitrogen Positope R900-40. ELISA: We sµggest a 1:5,000-1:10,000 dilution. WESTERN BLOTS: We sµggest a 1:2,000 to 1:10,000 dilution. For immunohistochemistry, we sµggest a 1:500 dilution (see image). Please note that the investigator must determine the appropriate dilution for your particular application.

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.

1. Murshid, A., Srivastava, A., Kumar, R and J. F. Presley, 2006, Characterization of the localization and function of NECAP 1 in neurons. Journal of Neurochemistry 98 (6): 1746–1762._x000D_ 2. Martin EA, Muralidhar S, Wang Z, Cervantes DC, Basu R, Taylor MR, Hunter J, Cutforth T, Wilke SA, Ghosh A, Williams ME. The intellectual disability gene Kirrel3 regulates target-specific mossy fiber synapse development in the hippocampus Elife. 2015 Nov 17;4. pii: e09395. doi: 10.7554/eLife.09395