SARS-CoV-2 Spike NTD Protein, His Tag (BA.2/Omicron) (MALS verified)

SARS-CoV-2 Spike NTD, His Tag (BA.2/Omicron) (SPD-C522h) is expressed from human 293 cells (HEK293). It contains AA Ser 13 - Leu 303 (Accession # QHD43416.1(T19I, LPP24-26del, A27S, G142D, V213G)). The spike mutations are identified on the SARS-CoV-2 Omicron variant (Pango lineage: BA.2; GISAID clade: GRA; Nextstrain clade: 21L).

Product Specifications

Background

It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 34.6 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Expression Region

Ser 13 - Leu 303

Host

HEK 293

Target

Spike NTD

Conjugation

Unconjugated

Tag

C-10×His

Source

SARS-CoV-2

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized SARS-CoV-2 Spike NTD, His Tag (BA.2/Omicron) (Cat. No. SPD-C522h) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Spike NTD Neutralizing Antibody, Chimeric mAb, Human IgG1 (Cat. No. SPD-M121) with a linear range of 0.1-2 ng/mL (QC tested).