Anti-Hsp90 α Antibody

Mouse Monoclonal Antibody specific to Hsp90α

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-Hsp90α Mouse Monoclonal Antibody

Gene Name

HSP90AA1

Gene ID

9031

NCBI Gene ID

UniProt

P11501

Accession Number

NP_001103255.1

Cellular Locus

Cytoplasm, Melanosome

Host

Mouse

Reactivity

Human, Mouse, Rat, Rabbit, Bovine, Porcine, Chicken

Immunogen

Full-length Hsp90 purified from chicken brain

Target Antigen

Heat shock protein HSP 90-α

Target

Hsp90 α

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, IHC, IP, ELISA, AM

Field of Research

Heat Shock& Stress Proteins

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity which is essential for its chaperone activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate rUniProtKB:P07900}.

Additionnal Information

Immunoblotting: use at 1-5ug/mL. A band of ~90 kDa is detected. Immunoprecipitation: 5ug on 20ul Protein A-Sepharose + 100ul sample. These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Heat-shocked HeLa cell lysate.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human, mouse, rat, rabbit, bovine, porcine, and chicken Hsp90alpha (90 kDa).

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

Hsp90 and the 94 kDa glucose-regulated protein, Grp94, are major molecular chapeones of the cytosol and endoplasmic reticulum. In mammalian cells, there are at least two Hsp90 isoforms, Hsp90alpha and Hsp90beta, which are encoded by separate genes. All known members of the Hsp90 family are highly conserved, especially in the N-terminal and C-terminal regions. In the absence of stress, Hsp90 is an essential component of cellular processes such as hormone signaling and cell cycle control. Several regulatory proteins such as steroid receptors, cell cycle kinases and p53 have been identified as substrates of Hsp90.