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Ammonium chloride, for cell culture

Ammonium chloride, for cell culture (Salmiac, for cell culture) is a reagent that can be used in cell culture to provide a source of nitrogen. Ammonium chloride can be used as a heteropolar compound to regulate pH value, which can cause intracellular alkalination and metabolic acidosis, thus affecting the activity of enzymes and affecting the process of biological systems. Ammonium chloride acts as an autophagy inhibitor.Ammonium chloride is also a lysosome inhibitor.

Product Specifications

CAS Number

[12125-02-9]

Product Name Alternative

Salmiac, for cell culture

UNSPSC

12352200

Hazard Statement

H302, H319

Target

Autophagy; Biochemical Assay Reagents

Type

Biochemical Assay Reagents

Related Pathways

Autophagy; Others

Field of Research

Cancer

Purity

99.0

Solubility

H2O : ≥ 100 mg/mL

Smiles

N.Cl

Molecular Formula

NH4Cl

Molecular Weight

53.49

Precautions

H302, H319

References & Citations

[1]Yu C, et al. Lysosome dysfunction enhances oxidative stress-induced apoptosis through ubiquitinated protein accumulation in Hela cells. Anat Rec (Hoboken) . 2013 Jan;296 (1) :31-9. |[2]Huang X, et al. NH4Cl treatment prevents doxorubicin-induced myocardial dysfunction in vivo. Life Sci. 2019;227:94-100.|[3]Canning WM, Fields BN. Ammonium chloride prevents lytic growth of reovirus and helps to establish persistent infection in mouse L cells. Science. 1983;219 (4587) :987-988.

Shipping Conditions

Room Temperature

Storage Conditions

4°C (Powder, sealed storage, away from moisture)

Scientific Category

Biochemical Assay Reagents

Clinical Information

No Development Reported

Citation 01

Adv Sci (Weinh) . 2025 Aug 4:e04680.|Adv Sci (Weinh) . 2025 Nov 7:e18323.|Aquaculture. 2025 Oct 15.|bioRxiv. 2024 November 19.|bioRxiv. 2025 Sep 6.|Cancer Lett. 2025 Apr 10:217715.|Cell Rep. 2025 Feb 19;44 (3) :115339.|Eur J Med Chem. 2025 Jun 14:296:117871.|MedComm (2020) . 2025 Aug 31;6 (9) :e70353.|PLoS Pathog. 2025 Jul 16;21 (7) :e1013344.|SSRN. 2025 Sep 30.|Acta Pharm Sin B. 2021 Sep;11 (9) :2850-2858.|Adv Sci (Weinh) . 2025 Jun 29:e09533.|Adv Sci (Weinh) . 2025 Oct 22:e07602.|Antiviral Res. 2023 Jun:214:105606.|Aquaculture. 2025 Feb 15.|Autophagy. 2025 Aug 28:1-15.|Autophagy. 2025 Aug;21 (8) :1644-1661.|Biochem Pharmacol. 2018 Apr:150:280-292.|Biochim Biophys Acta Mol Cell Biol Lipids. 2025 Apr 26:159618.|Cell Death Differ. 2025 Apr 8.|Cell Death Differ. 2025 Jun;32 (6) :1041-1057.|Cell Death Differ. 2025 May 20.|Cell Death Dis. 2024 Sep 11;15 (9) :665.|Cell Rep. 2025 Apr 2;44 (4) :115489.|iScience. 2024 Mar 12;27 (4) :109497.|J Immunol. 2025 May 30:vkaf118.|J Leukoc Biol. 2025 Jun 23:qiaf095.|J Virol. 2025 May 22:e0034725.|Nat Commun. 2024 Jul 18;15 (1) :6043.|Nat Commun. 2025 Jan 2;16 (1) :339.|PLoS Pathog. 2024 Feb 14;20 (2) :e1011981.|Research Square Preprint. 2023 Jun 22.|Signal Transduct Target Ther. 2024 Dec 30;9 (1) :367.|Vet Res. 2025 Apr 23;56 (1) :87.|Vet Res. 2025 Feb 28;56 (1) :46.|Virol J. 2022 Sep 20;19 (1) :151.|Viruses. 2019 Dec 30;12 (1) :43.

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