Recombinant Human Interleukin-4 (IL4) (Active)

Product Specifications

Product Name Alternative

Interleukin-4; IL-4; B-cell stimulatory factor 1 (BSF-1) ; Binetrakin; Lymphocyte stimulatory factor 1; Pitrakinra

Abbreviation

Recombinant Human IL4 protein (Active)

Gene Name

IL4

UniProt

P05112

Expression Region

25-153aa

Organism

Homo sapiens (Human)

Target Sequence

HKCDITLQEIIKTLNSLTEQKTLCTELTVTDIFAASKNTTEKETFCRAATVLRQFYSHHEKDTRCLGATAQQFHRHKQLIRFLKRLDRNLWGLAGLNSCPVKEANQSTLENFLERLKTIMREKYSKCSS

Tag

C-terminal 10xHis-tagged

Type

Active Protein & In Stock Protein

Source

Mammalian cell

Field of Research

Immunology

Relevance

Cytokine secreted primarily by mast cells, T-cells, eosinophils, and basophils that plays a role in regulating antibody production, hematopoiesis and inflammation, and the development of effector T-cell responses .

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Yes

Bioactivity

The ED50 as determined by the dose-dependent stimulation of the proliferation of TF-1 cells is 1.864-4.949 ng/mL.

Form

Lyophilized powder

Buffer

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Molecular Weight

16.3 kDa

References & Citations

An IL-4 signalling axis in bone marrow drives pro-tumorigenic myelopoiesis. LaMarche N.M., Hegde S., Park M.D., Maier B.B., Troncoso L., Le Berichel J., Hamon P., Belabed M., Mattiuz R., Merad M. Nature 625:166-174 (2024)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.