SARS-CoV-2 Spike Protein Total Antibody ELISA Kit

This kit recognizes SARS-CoV-2 Spike Protein Total Antibody in samples.No significant cross-reactivity or interference between Human SARS-CoV-2 Neutralization Antibody and analogues was observed

COVID 19 Spike Protein Total Antibody; Spike Protein Total Antibody; Spike Protein Antibody; SP Total Antibody; SP Antibody; SARS-CoV-2 Spike (RBD) Tab、2019-nCoV Spike (RBD) Tab、COVID-19 Spike (RBD) Tab

This ELISA kit uses the Sandwich-Ag ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with recombinant SARS-CoV-2 Spike Protein. After adding samples (or controls) to wells, the total antibodies against SARS-CoV-2 Spike Protein will combine with the pre-coated SARS-CoV-2 Spike Protein. After washing completely, add Horseradish Peroxidase (HRP) conjugated SARS-CoV-2 Spike Protein to develop the antigen-antibody-HRP conjugated antigen complex. Free components are washed away. The substrate solution is added to each well. Only those wells that contain SARS-CoV-2 Spike Protein, antibodies against SARS-CoV-2 Spike Protein and HRP conjugated SARS-CoV-2 Spike Protein will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. Compared with the CUT OFF value to judge whether SARS-CoV-2 Spike Protein total antibody exists in the tested samples or not.