Anti-GFAP Antibody

Mouse Monoclonal Antibody specific to GFAP

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

GFAP

Gene Name

GFAP

NCBI Gene ID

UniProt

P14136

Cellular Locus

Cytoplasm

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to aa 411-422 (KTVEMRDGEVIK) of human GFAP. This sequence is 100

Target Antigen

Glial fibrillary acidic protein

Target

GFAP

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, IHC, ICC/IF

Field of Research

Neuroscience

Purification Method

Purified by Protein G affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

Additionnal Information

Immunoblotting: use at 1-4ug/mL. A band of ~50kDa is detected. Immunofluorescence: use at 10ug/mL. These are recommended concentrations. Endusers should determine optimal concentrations for their application.

Storage Conditions

This product is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human, mouse and rat GFAP. It cross-reacts with GFAP- R416W and other GFAP mutant proteins.

Formulation

PBS, pH 7.4, 0.1% sodium azide, 50% glycerol.

Buffer pH

pH 7.4

Target Background

Glial fibrillary acidic protein (GFAP) is a major structural component of astrocytes. Phosphorylation of GFAP, and its association with annexin II-p2 and S-100, regulates GFAP polymerization. One of the first events that occurs during astrocyte proliferation is increased GFAP expression. Antibodies to GFAP have been detected in individuals with dementia, although the significance of this has not been conclusively determined.