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Starch Debranching Enzyme (DBE) Activity Colorimetric Assay Kit

Product Specifications

Type

Colorimetric method

Field of Research

Plant Physiology Series

Assay Principle

Starch Debranching Enzyme (DBE) can specifically split the α-1, 6-glucoside bonds of amylopectin. The resulting reducing sugars were determined by 3, 5-dinitrosalicylic acid method, and their light absorption values were measured at 540 nm. Then the activity of starch debranching enzyme was calculated indirectly.

Assay Performance Time

1 h 30 min

Sample Type

Plant tissue

Detection Range

0.9-18.0 U/g

Sensitivity

0.9 U/g

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at 2-8°C for 12 months with shading light.

Inter CV

1.0-1.4%

Intra CV

0.7-1.2%

Detection Instrument

Microplate reader

Documents

Product Datasheet

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