Starch Debranching Enzyme (DBE) Activity Colorimetric Assay Kit
Product Specifications
Type
Colorimetric method
Field of Research
Plant Physiology Series
Assay Principle
Starch Debranching Enzyme (DBE) can specifically split the α-1, 6-glucoside bonds of amylopectin. The resulting reducing sugars were determined by 3, 5-dinitrosalicylic acid method, and their light absorption values were measured at 540 nm. Then the activity of starch debranching enzyme was calculated indirectly.
Assay Performance Time
1 h 30 min
Sample Type
Plant tissue
Detection Range
0.9-18.0 U/g
Sensitivity
0.9 U/g
Shipping Conditions
Ice bag
Storage Conditions
This product can be stored at 2-8°C for 12 months with shading light.
Inter CV
1.0-1.4%
Intra CV
0.7-1.2%
Detection Instrument
Microplate reader
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