Anti-PSD95 Antibody

Cell membrane, Cell junction, synapse, postsynaptic density, Cell junction, synapse, Cytoplasm, Cell projection, axon, Cell projection, dendritic spine, Cell projection, dendrite, Cell junction, synapse, presynapse

Postsynaptic scaffolding protein that plays a critical role in synaptogenesis and synaptic plasticity by providing a platform for the postsynaptic clustering of crucial synaptic proteins (PubMed:15317815, PubMed:15358863, PubMed:19596852, PubMed:23300088, PubMed:26679993). Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B (By similarity). Also regulates AMPA-type glutamate receptor (AMPAR) immobilization at postsynaptic density keeping the channels in an activated state in the presence of glutamate and preventing synaptic depression (PubMed:19596852).Under basal conditions, cooperates with FYN to stabilize palmitoyltransferase ZDHHC5 at the synaptic membrane through FYN-mediated phosphorylation of ZDHHC5 and its subsequent inhibition of association with endocytic proteins (By similarity). {UniProtKB:P78352, UniProtKB:Q62108, PubMed:15317815, PubMed:15358863, PubMed:19596852, PubMed:23300088, PubMed:26679993}.

Immunoblotting: use at 4ug/mL. A band of ~100 kDa is detected. <br><br>Immunocytochemistry: use at 3-15ug/mL <br><br>These are recommended concentrations. User should determine optimal concentrations for their application. <br><br>Positive control: Rat brain tissue

Postsynaptic Density Protein 95 (PSD95), also known as Synapse Associated Protein 90kDa, is a member of the membrane- associated guanylate kinase family of proteins. PSD95 is a scaffolding protein involved in the assembly and function of the postsynaptic density complex. This family of proteins consists of N-terminal variable segments followed by three amino-terminal PDZ domains, an upstream SH3 domain, and an inactive carboxy-terminal guanylate kinase domain. The first and second PDZ domains localize NMDA receptors and K+ channels to synapses, and the third binds to neuroligins, neuronal cell adhesion molecules. PSD95 also binds to neuronal nitric oxide synthase. PSD95 is implicated in experience-dependent plasticity and plays an indispensable role in learning. Mutations in PSD95 are associated with autism.