Anti-Ezrin (Phospho-Tyr353) Antibody

Rabbit Polyclonal Antibody specific to Ezrin (Phospho-Tyr353)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-Ezrin (Phospho-Tyr353) Rabbit Polyclonal Antibody

Gene Name

EZR

Gene ID

123900

NCBI Gene ID

UniProt

P15311

Cellular Locus

Apical cell membrane, Cell projection, Cell projection, microvillus membrane, Cell projection, ruffle membrane, Cytoplasm, cell cortex, Cytoplasm, cytoskeleton, Cell projection, microvillus

Host

Rabbit

Reactivity

Human

Immunogen

Peptide sequence that includes phosphorylation site of tyrosine 353 (Q-D-Y(p)-E-E) derived from human Ezrin and conjugated to KLH.

Target Antigen

Ezrin

Target

Ezrin (Phospho-Tyr353)

Clonality

Polyclonal

Type

Antibody

Applications

WB, IHC

Field of Research

Phosphospecific Antibodies

Purification Method

Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis. {PubMed:17881735, PubMed:18270268, PubMed:19111582}.

Additionnal Information

Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~81kDa is detected. Immunohistochemistry: use at dilution of 1:50- 1:100. These are recommended working dilutions. End user should determine optimal dilutions for their application.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody detects endogenous human Ezrin only when phosphorylated at tyrosine 353.Accession no.: P15311, NP_001104547.1

Formulation

PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Buffer pH

pH 7.4

Target Background

Ezrin (also known as cytovillin or villin-2) is encoded by the EZR gene. The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. It plays a key role in cell surface structure adhesion, migration, and organization. The N-terminal FERM domain strongly binds sodium-hydrogen exchanger regulatory factor (NHERF) proteins (involving long-range allostery). The C-terminal ERM domain binds to actin, phosphatidylinositol(4,5)bis-phosphate (PIP2) and membrane proteins like CD44 and ICAM-2.