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Nitrite Reductase (NiR) Activity Colorimetric Assay Kit

Product Specifications

Type

Colorimetric method

Field of Research

Plant Physiology Series

Assay Principle

Nitrite reductase (NiR) in the sample can reduce NO2- to NO, so that the NO2- involved in the diazotization reaction to produce purplish red compounds is reduced, that is, the change of absorbance value at 540 nm can reflect the activity of the sample nitrite reductase.

Assay Performance Time

1 h 30 min

Sample Type

Plant tissue

Detection Range

0.045-9 μmol/h/g

Sensitivity

0.045 μmol/h/g

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at 2-8°C for 12 months with shading light.

Inter CV

3.5-7.7%

Intra CV

0.6-1.8%

Detection Instrument

Microplate reader

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