Nitrite Reductase (NiR) Activity Colorimetric Assay Kit
Product Specifications
Type
Colorimetric method
Field of Research
Plant Physiology Series
Assay Principle
Nitrite reductase (NiR) in the sample can reduce NO2- to NO, so that the NO2- involved in the diazotization reaction to produce purplish red compounds is reduced, that is, the change of absorbance value at 540 nm can reflect the activity of the sample nitrite reductase.
Assay Performance Time
1 h 30 min
Sample Type
Plant tissue
Detection Range
0.045-9 μmol/h/g
Sensitivity
0.045 μmol/h/g
Shipping Conditions
Ice bag
Storage Conditions
This product can be stored at 2-8°C for 12 months with shading light.
Inter CV
3.5-7.7%
Intra CV
0.6-1.8%
Detection Instrument
Microplate reader
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75-195 100 µL
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