Recombinant Porcine transmissible gastroenteritis coronavirus Nucleoprotein (N)
Product Specifications
Product Name Alternative
(Nucleocapsid protein) (NC) (Protein N)
Abbreviation
Recombinant Porcine transmissible gastroenteritis coronavirus N protein
Gene Name
N
UniProt
P04134
Expression Region
1-382aa
Organism
Porcine transmissible gastroenteritis coronavirus (strain Purdue) (TGEV)
Target Sequence
MANQGQRVSWGDESTKTRGRSNSRGRKNNNIPLSFFNPITLQQGSKFWNLCPRDFVPKGIGNRDQQIGYWNRQTRYRMVKGQRKELPERWFFYYLGTGPHADAKFKDKLDGVVWVAKDGAMNKPTTLGSRGANNESKALKFDGKVPGEFQLEVNQSRDNSRSRSQSRSRSRNRSQSRGRQQFNNKKDDSVEQAVLAALKKLGVDTEKQQQRSRSKSKERSNSKTRDTTPKNENKHTWKRTAGKGDVTRFYGARSSSANFGDTDLVANGSSAKHYPQLAECVPSVSSILFGSYWTSKEDGDQIEVTFTHKYHLPKDDPKTGQFLQQINAYARPSEVAKEQRKRKSRSKSAERSEQDVVPDALIENYTDVFDDTQVEIIDEVTN
Tag
N-terminal 10xHis-tagged and C-terminal Myc-tagged
Type
Developed Protein
Source
E.coli
Field of Research
Others
Relevance
Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication.
Endotoxin
Not test
Purity
Greater than 90% as determined by SDS-PAGE.
Activity
Not Test
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Molecular Weight
51.0 kDa
References & Citations
"Phosphorylation and subcellular localization of transmissible gastroenteritis virus nucleocapsid protein in infected cells." Calvo E., Escors D., Lopez J.A., Gonzalez J.M., Alvarez A., Arza E., Enjuanes L. J. Gen. Virol. 86:2255-2267 (2005)
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Product MSDS
https://www.cusabio.com/msds/12933937/
Protein Length
Full Length
Available Sizes
Curated Selection
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