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Biotinylated Human HLA-A*02:01&B2M&Survivin (TLPPAWQPFL) Complex Protein (Monomer, MALS verified)

Survivin, also known as BIRC5 and EPR-1, is a member of the inhibitor of apoptosis (IAP) family, which encodes negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but birc5 encodes proteins with only a single BIR domain. The Human HLA-A*0201 Survivin (TLPPAWQPFL) complex protein is a complex of HLA-A*0201 of the MHC Class I, B2M and TLPPAWQPFL peptide of the Survivin.

Product Specifications

Background

Survivin, also known as BIRC5 and EPR-1, is a member of the inhibitor of apoptosis (IAP) family, which encodes negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but birc5 encodes proteins with only a single BIR domain. The Human HLA-A*0201 Survivin (TLPPAWQPFL) complex protein is a complex of HLA-A*0201 of the MHC Class I, B2M and TLPPAWQPFL peptide of the Survivin.

Specifications

Biotinylated Human HLA-A*02:01&B2M&Survivin (TLPPAWQPFL) Complex Protein is produced by co-expression of HLA and B2M loaded with Survivin peptide. This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 36.3 kDa and 13.9 kDa. The protein migrates as 40-43 kDa and 12 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

AAA59606.1 (HLA-A*02:01) & P61769-1 (B2M) & TLPPAWQPFL

Expression Region

Gly 25 - Ile 308 (HLA-A*02:01) & Ile 21 - Met 119 (B2M) & TLPPAWQPFL peptide

Host

HEK293

Target

HLA-A*0201 & B2M & Survivin (TLPPAWQPFL)

Conjugation

Biotin-labeled

Tag

C-10xHis & C-Avi

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Biotinylated Human HLA-A*02:01&B2M&Survivin (TLPPAWQPFL) Complex Protein (Cat. No. HLN-H82E5) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.5-8 ng/mL (QC tested).

Format

Powder

Buffer

PBS, pH7.4

Additives

Trehalose

Molecular Weight

36.3 kDa and 13.9 kDa

Additionnal Information

Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments

Shipping Conditions

RT

Storage Conditions

-20°C

Package Size

200ug*1

Host or Source

HEK293

Species

Human

Protein ID

AAA59606.1 (HLA-A*02:01) & P61769-1 (B2M) & TLPPAWQPFL

Preservative

Trehalose

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