Recombinant Avian infectious bronchitis virus Non-structural protein 5a (5a)

Product Specifications

Product Name Alternative

(ns5a) (Accessory protein 5a)

Abbreviation

Recombinant Avian infectious bronchitis virus Non-structural protein 5a

Gene Name

UniProt

Q5I5X4

Expression Region

1-65aa

Organism

Avian infectious bronchitis virus (strain M41) (IBV)

Target Sequence

MKWLTSFVRAVISCYKPLLLTQLRVLDRLILDHGPKHILTCVRCVILFQLDLVYRLAYTPTQSLV

Tag

N-terminal 6xHis-SUMO-tagged

Type

In Stock Protein

Source

E.coli

Field of Research

Microbiology

Endotoxin

Not test

Purity

Greater than 85% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Molecular Weight

20.5 kDa

References & Citations

"Comparisons of envelope through 5B sequences of infectious bronchitis coronaviruses indicates recombination occurs in the envelope and membrane genes." Brooks J.E., Rainer A.C., Parr R.L., Woolcock P., Hoerr F., Collisson E.W. Virus Res. 100:191-198 (2004)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.