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Biotinylated Human IL-7 R alpha & IL-2 R gamma Protein, His, Avitag™&Tag Free (MALS verified)

Selected Variant

ILA-H82W3-100ug

Size: 100 µg

The protein encoded by this gene is a receptor for interleukin 7 (IL7) . The function of this receptor requires the interleukin 2 receptor, gamma chain (IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukins 2, 4, 7, 9, and 15. This protein has been shown to play a critical role in V (D) J recombination during lymphocyte development. Defects in this gene may be associated with severe combined immunodeficiency (SCID) . Alternatively spliced transcript variants have been found.

Product Specifications

Background

The protein encoded by this gene is a receptor for interleukin 7 (IL7) . The function of this receptor requires the interleukin 2 receptor, gamma chain (IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukins 2, 4, 7, 9, and 15. This protein has been shown to play a critical role in V (D) J recombination during lymphocyte development. Defects in this gene may be associated with severe combined immunodeficiency (SCID) . Alternatively spliced transcript variants have been found.

Specifications

Biotinylated Human IL-7 R alpha & IL-2 R gamma Protein, produced by co-expression of IL-7 R alpha and IL-2 R gamma, has a calculated MW of 32.2 kDa (IL-7 R alpha) & 31.2 kDa (IL-2 R gamma) . Subunit IL-7 R alpha is fused with a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) and subunit IL-2 R gamma contains no tag at the C-terminus. The protein migrates as 43-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

P16871-1 & P31785-1

Expression Region

Glu 21 - Asp 239 & Leu 23 - Asn 254

Host

HEK293

Target

IL-7 R alpha & IL-2 R gamma

Conjugation

Biotin-labeled

Tag

C-10xHis & C-Avi | Native

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Human IL-7, Fc Tag (Cat. No. IL7-H5253) at 2 μg/mL (100 μL/well) can bind Biotinylated Human IL-7 R alpha & IL-2 R gamma Protein, His,Avitag&Tag Free (Cat. No. ILA-H82W3) with a linear range of 0.1-2 ng/mL (QC tested).

Format

Powder

Buffer

PBS, pH7.4

Additives

Trehalose

Molecular Weight

32.2 kDa & 31.2 kDa

Additionnal Information

Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments

Shipping Conditions

RT

Storage Conditions

-20°C

Package Size

100ug*1

Host or Source

HEK293

Species

Human

Protein ID

P16871-1 & P31785-1

Preservative

Trehalose

Price

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