Peroxidase Signal Enhancer Kit

Mixing of reagent: To five ml buffer (reagent B) in a test tube, add one drop of concentrated Enhancing reagent (reagent E) mix well. This ready to use reagent is good for 4-6 hours. The unused solution can be discarded according to city, county, state, province or country’s regulations. Application: IHC, ICC 1. After the peroxidase reaction, wash slide with buffer 5-7X, (this buffer should not contain any sodium azide because peroxidase is inactivated) and distilled/ deionized water 2X. 2. Add few drops of ready to use enhancing reagent for 1-2 minutes and discard. Washing of slide is not required. 3. Add few drops of ready to use AEC reagent, or DAB reagent, follow instructions as outlined for these chromogens.